Preparation of biokit to treatment the Petrol spots in water

Microorganism isolates used in plant protection were obtained from the College of Agriculture / University of Baghdad. Its purity was assured. The microbial biokit was prepared through using several unsatisfactory and environmentally friendly isolate. The bioactivity of biokit were studied in laboratory and showed susceptibility in removing contaminants. The results showed that the biomass of the isolates prior to the loading process on a medium containing crude oil as a single carbon source of 5 g/ L, which indicates growth ability and efficiency in the decomposition of pollutants when increase the isolates biomass more than 3 g / L, as well as the appearance of turbidity in the media compared to the control sample (media without oil), and obtained bioemulsifier with a concentration of 2.3 g / L. The chemical analysis of the samples under study was carried out using Gas Chromatography (GC) and GC-MS compared with a non-treatment (control). The results showed the appearance of 43 topographic peaks each indicating a chemical compound in the control sample, while a number of these peaks disappeared and a space was reduced in other peaks that refers to the concentration of compounds, in the biomass sample, and reached the number of peaks in 26, indicating the efficiency of the biokit in the oil decomposition and dismantling of hydrocarbons. The increase in the number of live microorganisms and the incubation period is more than 28 days by increasing the growth intensity in the third week may be due to decomposing oil compounds and their exploitation in nutrition, where crude oil provides a source of carbon and energy for microorganism’s isolates. In other results showed that the mass of crude oil was converted to a mass of gel and emulsification compared to the control sample after seven days period at a temperature of 28± 2°C, Which is characterized by low toxicity and easy decomposition and reduces the surface tension of hydrocarbons and makes them more solubility and preparation for the dismantling of life and increase the rate of disintegration. The biokit was tested for retention in storage conditions for a period of one year. The results showed that the viability of the biokit was effectively compared to the isolates No.20 Journal of Petroleum Research & Studies (JPR&S) 2 at alone prior to the production of the biokit, which showed poor storage capacity. We conclude that biomass has the potential to treat hydrocarbons (oil contaminants) in the environment.


No.20 Journal of Petroleum Research & Studies (JPR&S)
3 laboratories in western countries have used these microorganisms extensively in treating oil stains in the seas and oceans that's caused by leaching of oil tankers [4].
A number of microorganisms Isolated and they have proved a highly efficient in the degradation of different types of hydrocarbons; their species are returned to bacteria, fungi and yeast. Biodegradation has many benefits, where it is an inexpensive method and does not require complex equipment; in addition it is a cheap method compared to other treatment methods.
Microorganism are able to exploit carbon compounds in oil as a source of energy [5].
So, this study aimed to produce local kit that are safe and friendly to environmental, composed from several microorganism isolates that are capable of degraded crude oil by loading them on chemical salts to increase their efficiency in degradation and reducing oil pollution in the water, as well as increase the storage period for a relatively long time compared to the global product with low cost and ease production.

1-Micro-organism that analyze hydrocarbons:
Microorganism isolates were obtained from the Department of Plant Protection, Faculty of Agriculture/ University of Baghdad which identified by the same Department. Their purity was verified using some preliminary and biochemical tests and save until being use.

2-Preparation of Culture media:
Mineral Salt Medium (MSM) which used for growth of microorganism isolate according to [6], Modified Mineral Salt Medium (MMSM) used to encourage oil emulsion process which leads to oil degradation this media prepared according to [7], Lactose-broth used to activate the preserved isolate and check its purity [8], also Nutrient Agar was used for growth of microorganism isolate, this Nutrient Agar was made by HIMEDIA Indian company and prepared according to manufacturer instructions.
Take the 100 g of CaCO 3 and sterilized by oven under 160º for one hour and left to cool, and then distributed in a shallow pot sterile and 100 ml from fermentation media that prepared previously, which contain the mixed microorganism at 48 age were put. The fermentation media was prepared according to [9]. Pots that are containing CaCO 3 and fermentation media was The Microorganism density was calculated in 1 ml according to [10].

C. Estimation of the amount of bioemulsions produced by Microorganism
The sample of crude oil was obtained from Kirkuk oil field and has the following characteristic in table (1).

D. The a viability of the biokit for storage.
After the completion of the production process for biokit it was store in sterilize container under lab conditions, after one year the density of microorganisms was estimated in 1g of the kit, in addition to study the morphological changes in crude oil degraded by the action of biokit through the incubation of the kit for one week at room temperature and recording the morphological changes.

4-Estimation of the quantitative loss of crude oil as a result of use the biokit a. by using the Non-specialized chemical methods.
Test the viability of the kit to degrade the crude oil through:

2-
The ease of production of biokit at relatively low costs and high productivity and relatively with short periods of time.

3-
The new microbial kit has effective bioactivity compared to the foreign product.
4-Production materials are locally available, friendly for environmental and public health and relatively inexpensive.