Poly aromatic hydrocarbons degradation by lignin peroxidase produced from local isolate Aspergillus terreus SG777

Sahar Ghazi Imran; Nada S. Razuqi; Ghazi M. Aziz

doi:10.52716/jprs.v8i2.240

Authors

Sahar Ghazi Imran The Ministry of Science & Technology
Nada S. Razuqi The Ministry of Science & Technology
Ghazi M. Aziz The Ministry of Science & Technology

DOI:

https://doi.org/10.52716/jprs.v8i2.240

Keywords:

Environment and Safety in Oil Industries, Aspergillus, lignin peroxidase, poly aromatic hydrocarbons

Abstract

Lignin peroxidase produced from A. terreus SG777 precipitated with 80% ammonium sulfate
saturation then desalting by dialysis against Tris buffer solution and partially purified by ion
exchange chromatography using DEAE- cellulose.The specific activity reached 56.67U/mg with
purification fold was 5.4 times and 13.7% recovery. The optimum pH for activity and stability was
(3). The maximum activity was observed at 40°C and the enzyme maintained the activity when it
was incubated at (25-35)˚C.
Degradation of poly aromatic hydrocarbons when incubated with 65.51 U/ml lignin peroxidase
at (40°C) for 4 hr compared with the Refinery treatment. Results showed (97.54%) removal
efficiency of Di benzo (a, h) anthrancene by lignin peroxidase, while only (54.37%) was treated by
Refinery treatment. Flourene and anthrancene degradation (95.98%) and (83.51%) respectively,
while the flourene and anthrancene degradation treated by Refinery treatment only 84.51% and
(46.83%) respectively.
The aim of this study was degradation of poly aromatic hydrocarbons by using partial
purification lignin peroxidase.