A study of Using a Phyto-nanosynthesis of Silver and Portulaca Oleracea Plant Extracts in Petroleum Spots Treatment


  • Muthik Abd Guda Department of Ecology, Faculty of Science, University of Kufa, Najaf, Iraq
  • Anam A. Tsear Department of Ecology, Faculty of Science, University of Kufa, Najaf, Iraq
  • Laith Saheb Department of Ecology, Faculty of Science, University of Kufa, Najaf, Iraq.




Portulaca oleracea, Normal coagulation, Turbidity, COD, TOC


Petroleum spots has become a major global problem not only in terms of its increase, but also how it is treated, recycled or utilized. It contains suspended and dissolved solids, hydrocarbons and many types of organic matter and heavy metals. The reuse of petroleum spots faces the problem of removing organic pollutant compounds before discharging them into any natural stream. in this study, natural nano –coagulant material was used and their efficiency in removing turbidity, Total Organic Carbon (TOC) and Chemical Oxygen Demand (COD) from petroleum spots were compared. The study was conducted in the laboratories of the Ecology Sciences Department, University of Kufa, Iraq in 2022. By synthesizing Phyto-nanosilver as a Phyto-nano silver from Leaves of Portulaca oleracea plant extracts used to reduce turbidity and organic pollutants of the petroleum spots. Jar test experiments showed that 0.5 mg / L Phyto-nanosilver dose can remove 95.5 % of (COD), 87.3% of TOC and 85.2 % from turbidity. Also, removal of P. oleracea plant extracts in the 1 and 0.5 mg/L dose can removed turbidity, COD and TOC content of 91.9%, 87.5 % and 82.4% respectively of the petroleum spots. Moreover, when using the nano-sliver only, removal of TOC, COD, and turbidity would reach 75.3%, 80.4%, and 81.3 %, respectively. The results of the research showed the efficiency of Phyto-nano silver materials in treating and depositing pollutants without harming the environment.


A. T. Ali, M. A. Guda, A. I. Oraibi, I. K. Salih, A. H. Shather, A. T. Abd Ali, A. L. Azzawi, and H. A. Almashhadani, "Fe3O4 supported [Cu (ii)(met)(pro-H) 2] complex as a novel nanomagnetic catalytic system for room temperature C–O coupling reactions", RSC advances vol. 13 No. 32, pp. 22538-22548, 2023. https://doi.org/10.1039/D3RA03509C

D. M. Husayn, and M. A. Guda, "Effect of zinc oxide nanoparticles on biomarkers of chlorophyll and carotene in some wild plants", AIP Conference Proceedings, vol. 2787, no. 1, 2023. https://doi.org/10.1063/5.0148197

D. M. Husayn, and M. A. Guda, "Response of some wild plants in antioxidant enzymes by zinc oxide nanoparticles", AIP Conference Proceedings, vol. 2787 no. 1, 2023. https://doi.org/10.1063/5.0148199

M. O. Hammad, and M. A. Guda, "Effect of Phytosynthesis silver oxide nanoparticles on multidrug-resistant Klebsiella pneumonia isolated from children", Eur. Chem. Bull, vol. 12 no. 1, pp.1655-1669, 2023.

A. H. K. Al-Hasnawi, and M. A. Guda, "Comet Assay Technique Value as a Response on Zinc Oxide Nanoparticales in Trigonella foenum-graecum Plant", International Journal of Advanced Multidisciplinary Research and Studies Search, vol. 3, no. 4, pp. 809-814, 2023.

A. Y. Rahi and M. A. Guda, "Effect Biosynthesis of fenugreek leaves nanomaterial on some plant's germination using saline water", Revis Bionatura, vol. 8, no. 2, pp. 1837-1746, 2023.

O. A. R. Owied, M. A. M. Guda, H. I. Taher, and M. A. A. Abdulhussein, "Plants Anatomical Engineered by Nanomaterials", Revis Bionatura, vol. 8, no. 2, pp. 44-48, 2023. Volume 8 / Issue 2 / 44 / http://dx.doi.org/10.2931/RB/2023.08.02.44

M. A. Guda, and E. A. R. A. Semysim, "Response of A Medicinal Plant Peganum Harmala to Iron Oxide Nanoparticles F3O4 (Nps)", Journal of Pharmaceutical Negative Results, vol. 13, vol. 13, special issue 08, pp. 289-308, 2022. https://doi.org/10.47750/pnr.2022.13.S08.123

M. M. Alabassi, M. A. Guda, and M. A. Muhammed, "The removal efficiency of Phyto-nano silver produced from Phragmites communis, Schanginia aegyptiaca and Portulaca oleracea in petroleum spots treatment", International Journal of Aquatic Biology, vol. 10, no. 2, pp. 181-186, 2022.

M. A. Guda, N. H. Mutlag, A. A. Tsear. "The use of Atriplex nummularia plant as the hyperaccumulators of silver", In AIP Conference Proceedings, vol. 2290, no. 1, AIP Publishing, 2020. https://doi.org/10.1063/5.0027565

Z. H. Thajeel, M. A. Guda, N. H. Mutlag, and B. S. Jabbar, "Investigation the effect of irrigation with magnetized saline water to improve the vegetative growth and the anatomical characteristics of the tomato plant (Lycopersicon Esculentum L.)", International Journal of Agricultural & Statistical Sciences, vol. 16, no. Supp-01, pp. 1551-1557, 2020.

G. L. Hakeem, M. A. Guda, M. M. Alabassi, A. J. T. Altamimi, and H. A. N. Alhadrawi, "Use of wild plant species as indicator of some heavy metals in the soil of General Company for tire industry in Najaf Governorate", IOP Conference Series: Materials Science and Engineering, vol. 870, p. 012099, 2020. https://doi.org/10.1088/1757-899X/870/1/012099

A. H. Assi, “Non-Productive Time Reduction during Oil Wells Drilling Operations”, Journal of Petroleum Research and Studies", vol. 12, no. 3, pp. 34-50, September 2022. http://doi.org/10.52716/jprs.v12i3.541

J. T. Shakib, V. Kanani, and P. Pourafshary, "Nano-clays as additives for controlling filtration properties of water–bentonite suspensions", Journal of Petroleum Science and Engineering, vol. 138, pp. 257-264, 2016. https://doi.org/10.1016/j.petrol.2015.11.018

A. H. Assi, “The Effect of Micro and Nona Silica Addition on the Filtration and Mud Cake of Drilling Fluid”, Journal of Petroleum Research and Studies, vol. 13, no. 3, pp. 41-58, Sep. 2023. http://doi.org/10.52716/jprs.v13i3.694

B. Rajani, and S. Suresh, "Biosynthesis of ZnO/SiO2 nanocatalyst with palash leaves’ powder for treatment of petroleum refinery effluent", Resource-Efficient Technologies, vol. 3, no. 4, pp. 528-541, 2017. https://doi.org/10.1016/j.reffit.2017.08.004

M. A. Guda, S. J. Hadi, K. K. Alasedi, F. J. Alduhaidahawi, L. Saheb, and M. F. Ali, "Antioxidant enzyme response of medical plant Persian Fenugreek (Trigonella foenum-graecum L.) to irrigation with microwaves treated water", international journal of research in pharmaceutical sciences, vol. 12, no. 1, pp. 442 – 445, 2021.

Y. He, L. Qin, and H. Huang, “Calcium-enhanced retention of humic substances by carbon nanotube membranes: Mechanisms and implication”, Journal of Membrane Science, vol. 629, 119273, 2021. https://doi.org/10.1016/j.memsci.2021.119273

S. Guo, Y. Wan, X. Chen, and J. Luo, “Loose nanofiltration membrane custom-tailored for resource recovery”, Chemical Engineering Journal, vol. 409, 127376, 2021. https://doi.org/10.1016/j.cej.2020.127376

Z. Abdulhussein, Z. T. Al-sharify, M. Alzuraiji, H. Onyeaka, “Environmental Significance of Fouling on the Crude Oil Flow. A Comprehensive Review: Fouling on The Crude Oil Flow”, Journal of Engineering and Sustainable Development, vol. 27, No. 3, pp. 317-338, 2023.‏ https://doi.org/10.31272/jeasd.27.3.3




How to Cite

Guda, M. A.; Tsear, A. A. .; Saheb, L. . A Study of Using a Phyto-Nanosynthesis of Silver and Portulaca Oleracea Plant Extracts in Petroleum Spots Treatment. Journal of Petroleum Research and Studies 2024, 14, 138-149.