المعالجة الحيوية للتربة الملوثة بالهيدروكربونات في محطات الوقود باستخدام إنزيم ألكان هيدروكسيلاز المُنقّى من بكتيريا Pseudomonas Fluorescens

Hayfaa A. Mubarak; Ammar A. Abd Alamer; Assel A. Hadi; Ahmed Al-Salman; Aaya Q. Q. Khaleel

doi:10.52716/jprs.v15i3.964

Authors

Hayfaa A. Mubarak Department Chemical Engineering, College of Engineering, University of Babylon, Iraq
Ammar A. Abd Alamer General Company for Gas Filling and Services, Ministry of Oil, Baghdad, Iraq.
Assel A. Hadi Department of Medical Physics, College of Sciences, Al-Mustaqbal University, Babylon, Iraq.
Ahmed Al-Salman Department Chemical Engineering, College of Engineering, University of Babylon, Iraq.
Aaya Q. Q. Khaleel Department of Chemistry, College of Sciences, Al-Nahrain University, Baghdad, Iraq.

DOI:

https://doi.org/10.52716/jprs.v15i3.964

Keywords:

alkane hydroxylase, Pseudomonas spp., bioremediation.

Abstract

Petroleum product pollution of soil is a worldwide issue that threatens human and environmental health and destroys natural ecosystems. It also interferes with the ecosystem's ability to function properly. Thus, the necessity to clean up polluted locations is vital. Bioremediation is an economical and ecologically friendly approach. The ability of certain bacteria to proliferate in situations contaminated with oil makes bacterial alkane hydroxylases highly desirable for bioremediation applications. Additionally, this enzyme facilitates specific hydroxylation of chemically inert alkanes, which is useful for synthesizing expensive compounds like medicines. In this study with a high level of alkane hydroxylase at 40°C after 72 hours of incubation, Pseudomonas fluorescens was found to be the most effective alkane degrader, followed by Pseudomonas aeruginosa, which had a lower productivity for alkane hydroxylase. Upon reaching a peak after 96 hours with 67% degradation of the hydrocarbons, alkane hydroxylase at a concentration of 150 µg/ml removed 43% of the hydrophobic pollutant from the polluted soil following a 24-hour incubation period.

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