المعالجة الحيوية للتربة الملوثة بالهيدروكربونات في محطات الوقود باستخدام إنزيم ألكان هيدروكسيلاز المُنقّى من بكتيريا Pseudomonas Fluorescens

Hayfaa A. Mubarak; Ammar A. Abd Alamer; Assel A. Hadi; Ahmed Al-Salman; Aaya Q. Q. Khaleel

doi:10.52716/jprs.v15i3.964

المؤلفون

Hayfaa A. Mubarak Department Chemical Engineering, College of Engineering, University of Babylon, Iraq
Ammar A. Abd Alamer General Company for Gas Filling and Services, Ministry of Oil, Baghdad, Iraq.
Assel A. Hadi Department of Medical Physics, College of Sciences, Al-Mustaqbal University, Babylon, Iraq.
Ahmed Al-Salman Department Chemical Engineering, College of Engineering, University of Babylon, Iraq.
Aaya Q. Q. Khaleel Department of Chemistry, College of Sciences, Al-Nahrain University, Baghdad, Iraq.

DOI:

https://doi.org/10.52716/jprs.v15i3.964

الكلمات المفتاحية:

alkane hydroxylase, Pseudomonas spp., bioremediation

الملخص

إن قدرة بعض البكتيريا على التكاثر في المواقع الملوثة بالزيت تجعل هيدروكسيد الألكان البكتيري مرغوبًا للغاية في تطبيقات المعالجة الحيوية. بالإضافة إلى ذلك، يسهل هذا الإنزيم عملية الهدرجة المحددة للألكانات الخاملة كيميائيًا، وهو أمر مفيد في تصنيع مركبات باهظة الثمن مثل الأدوية. في هذه الدراسة مع مستوى عالٍ من هيدروكسيد الألكان عند 40 درجة مئوية بعد 72 ساعة من الحضانة، وجد أن الزائفة المتألقة هي أكثر مزيلات الألكان فعالية، تليها الزائفة الزنجارية، التي كانت لديها إنتاجية أقل لهيدروكسيد الألكان. عند الوصول إلى الذروة بعد 96 ساعة مع تحلل الهيدروكربونات بنسبة 67%، قام هيدروكسيلاز الألكان بتركيز 150 ميكروغرام/مل بإزالة 43 % من الملوثات الكارهة للماء من التربة الملوثة بعد فترة حضانة مدتها 24 ساعة.

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